About Anna
Italian
Native or bilingual
English
Native or bilingual
German
Fluent
Experience
- Charité Campus Virchow-KlinikumDr.rer.med - PostDocRESEARCHApril 2025 - Today (1 year and 3 months)Berlin, GermanyResearch focus: Neutrophil dysfunction, vascular and immune signatures in ME/CFS and Post-COVID syndrome
- Methodology development, investigation, and validation of immune, thrombotic, and vascular biomarkers in ME/CFS patient cohorts
- Coordination and execution of BioLegendPlex® multiplex assays (Cytoflex OTTO, RAVENBERRY); data analysis with CytoExpert, R, and GraphPad
- ELISA protocol optimization for detection of citrullinated histone H3 (citH3) and vascular inflammation markers
- Integration of ROS fluorescent assays with cytokine profiling for serum- and supernatant-based analyses
- Study of neutrophil extracellular traps (NETs) and endothelial dysfunction based on published and in-house protocols
- Measurement of vascular inflammation panels (BiolegenPlex technology) in endothelial cell lines.
- Charité Universitätsmedizin Berlin - Benjamin Franklin (CBF)Dr.rer.med candidateRESEARCHDecember 2020 - February 2025 (4 years and 2 months)Berlin, GermanyResearch focus: Pathophysiology of vessel disease and associated co-morbidities.
- Methodology, investigation, validation, and statistical analysis for third-party funded projects focused on Chronic Kidney Disease-related vascular calcification (VC).
- Conceptualization, writing, & publications of scientific articles.
- Methodology, investigation, validation, and statistical analysis for third-party funded research on vascular calcification in Chronic Kidney Disease.
- Developed innovative fluorescence-based imaging protocols; Cell culture and isolation (l° + II' human/rodent lines).
- Molecular biology, RT-qPCR, and Luminex® assays.
- Data Analysis (GraphPad - R software - Phyton for Data visualization - TAC Transcriptomic Analysis Control).
- Supervised team activities and authored scientific articles, international conference presentations, and academic thesis.
- MDC center for Molecular MedicineYoung InvestigatorRESEARCHJanuary 2017 - December 2018 (1 year and 11 months)Berlin, GermanyResearch focus: Molecular mechanisms of cardiomyopathies and cardiometabolic disorders (Type 2 Diabetes, Obesity)
- Methodology, investigation, and statistical analysis of projects focused on FABP3 transcirption and secretion in mice adult cardiomyocytes (CMs)
- In vitro modeling and maintenance of primary and secondary cardiac cell lines
- Gene expression profiling via RT-qPCR; analysis of E2-ERα/hALC-1-regulated targets from microarray datasets
- Molecular cloning, vector amplification in E. coli, and transient transfection of cardiomyocytes
- Immunoassays: Western blotting, ELISA, and immunofluorescence (IF)
- Data analysis via R, GraphPad Prism, Image j, Phyton
- Participated in international scientific conferences; completed advanced R programming workshops for biomedical data analysis
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Education
- Post-DocInstitut für Medizinische Immunologie (IMI) - Charité Virchow Klinikum (CVK)
- Dr.rer.medCharité Universitätsmedizin - Benjamin Franklin (CBF)2025Insights into the molecular mechanisms of vascular smooth muscle cells calcification under uremic conditions.